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KMID : 0380020060210040312
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Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 4 p.312 ~ p.316
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A Study on the Antimicrobial Effect of Ginkgo biloba Leaves Extracts according to Concentrations of Ethanol for staphylococcus aureus
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Lee In-Hwa
Shim Youn
Choi Seung-Hyun
Park Ju-Young
Han Sung-Woo
Song Jn-Young
Yuon Suk-Jin
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Abstract
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A optimal condition for the Ginkgo biloba extraction in ethanol and water binary solvent system has been proposed based on concentration of bilobalide and ginkgolide known as having a antimicrobial components in the range 5% to 70% ethanol in water at 80℃. Concentration of bilobalide as a single component of Ginkgo biloba leaves extract is the highest at the 60% ethanol and ginkgolide A and B is highest at 50% ethanol. Antimicrobial effect of Ginkgo biloba leaves extracts on the S. aureus was also examined by disc diffusion test and optical density test. In case of the disc diffusion test, the clean zone diameter was increased from 0.95 cm to 1.70 cm as ethanol concentration increased from 5 to 70%. However, over the 40% of ethanol concentration the antimicrobial effect was almost flat. Based on these results, we propose that the 40% of ethanol and 60% water solvent is most desirable for Ginkgo biloba extract considering vapor pressure problem in concentrating process after extraction. We introduced SEM and TEM to figure out the morphological change on the surface and inside body of S. aureus when Ginkgo biloba leaves extract was treated. After mixed with Ginkgo biloba leaves extract blast like blebs appeared on the surface of S. aureus cells and cell wall was not observed. From the these results, it seems that the Ginkgo biloba leaves extract including bilobalide and ginkgolide A, B prevent cell wall synthesis.
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KEYWORD
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Ginkgo biloba Leaves extract, antimicrobial effect, Staphylococcus aureus
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